Nucleic Acid Medicine Oligos
Nucleic Acid Medicines are therapeutic elements that are based on nucleic acids or closely related to compounds that treat disease. They work by preventing the expression of disease-causing proteins by targeting the gene directly. We offer CpG-ODN, ICON Probe, and NF-kB decoy oligos to aid in the development of the therapeutics.
CpG-ODN
CpG-ODN is available to customers worldwide.
We offer high quality TLR9 agonists and their controls for activating immune cells. This product is a reagent for research. It cannot be used for clinical purposes.
Product Details
Overview
Toll-like receptors (TLRs) recognize highly conserved structural motifs as one of the early innate immune responses to invading pathogens. TLR 9 distinguishes mammalian and bacterial DNA and induces activation of immune cells.
A synthetic oligonucleotide containing a CpG motif has the effect of stimulating the immune system, promoting the maturation and activation of antigen presenting cells, and the induction of Th1 and inflammatory cytokines.
- Immune stimulation of potent and specific human, mouse immune cells, and for animal experiments
- Offered in encapsulated vials in high purity
- Guaranteed endotoxin 0.5 EU / mg or less
- Easy handling
Product Details
TLR9 agonists of different classes have been shown to have different effects on cells. Please select the agonist that is best suited for your needs.
A-class TLR9 ligand: D35
- Induction of high type I IFN production
- Low activity against B cells
- It is useful for activation of pDC and induction of IFN-α from PBMC, activation of IFN-signal pathway
B-class TLR9 ligand: K3
- Stimulates B cells and activates TLR9-dependent NF-kB signal
- Low activity for induction of FN-α
- B-class is useful for activation of B cell, induction of IL-6 from human PBMC, activation of NF-kB signal pathway
CpG-ODN Activity evaluation
Example 1: Cytokine induction in human PBMC
Human IFN-a and IL-6 concentrations in culture supernatants were measured by ELISA for 24 hours after stimulation of human PBMC (1 x 106 cells) with 1 uM of each CpG. The bar graph shows the mean average ± SEM.
Example 2: Cytokine induction in mouse FL-DC
Human IFN-a aMurine IFN-a and IL-6 concentrations in the culture supernatant of the mouse bone marrow-derived FL-DC (2 × 10 6 cells) induced to differentiate with FLT 3 L were stimulated with 1 uM of each CpG for 24 hours and then measured by ELISA. The bar graph shows the mean average ± SEM.
This data is provided as a result of collaborative research with Professor Ken Ishii and Professor Daiki Aoshi, Adjuvant Development Project of National Institute of Pharmaceutical Sciences.
References:
1: Ishii KJ, Gursel I, Gursel M, Klinman DM. Immunotherapeutic utility of stimulatory and suppressive oligodeoxynucleotides.
Curr Opin Mol Ther. 2004 Apr; 6 (2): 166-74.
2: Verthelyi D, Ishii KJ , Gursel M, Takeshita F, Klinman DM. Human peripheral blood cells differentially recognize and respond to two distinct CPG motifs.
J Immunol. 2001 Feb 15; 166 (4): 2372-7.
RNA purification example include cultured cells, bacteria, plants, and yeast.
EconoSpin™ Empty Column
- Filled the desired carrier
- Used for preparing affinity columns
- Used for preparing ion exchange columns
- Application example: Fill the Ni agarose carrier and purify the His tagged protein
Frequently Asked Questions
Which kit of which maker corresponds to which kit?
Although the main purpose of EconoSpin is a spin column substitute included in a commercially available nucleic acid purification kit, it is difficult to conduct a conformity test for each of the various types of kits that are currently offered.
In order to test whether it corresponds to various kits, we have prepared a free sample. If you would like a sample, please contact us.
The DNA yield is low (cannot be obtained)?
EconoSpin may be lower in yield than dedicated columns of each kit’s genuine product due to its character as a substitute. In addition, there is a possibility not to adapt depending on the purification method of each kit and the type of buffer.
What is the length of DNA that can be recovered by EconoSpin?
The chain length that can be collected with high probability is 100 to 10000 bp.
What is the elution volume of EconoSpin?
Depending on the experiment, it is approximately 10 to 50 μl.
What is the storage temperature of EconoSpin?
It can be stored at room temperature.
Is there a buffer recipe for EconoSpin for RNA?
A buffer recipe is not provided for the RNA column. Thank you for your understanding.
What is the difference between with lid and without lid?
With lid, it is possible to prevent the liquid from popping out when centrifuging. Without lid allows work to be done efficiently because there is no need to open and close the lid.
Since the amount of silica membrane is the same in both cases, there is no difference in the amount of DNA recovered. For RNA, we only have lid.
